Quality Assurance

From Synthesis to Shipment

Every peptide in our catalog passes through a rigorous 9-stage quality control pipeline before it reaches your laboratory.

9-Stage Quality Control Pipeline

1
Stage 1 of 9

Raw Material Sourcing

Pharmaceutical-grade amino acids from qualified, audited suppliers. Every incoming lot is inspected against a certificate of conformance before acceptance into inventory.

2
Stage 2 of 9

Solid-Phase Synthesis

SPPS synthesis with Fmoc chemistry protocols on automated peptide synthesizers. Coupling efficiency is monitored at each residue addition to catch truncations early.

3
Stage 3 of 9

HPLC Purification

Reverse-phase HPLC isolates the target peptide from deletion sequences, side-chain modifications, and residual protecting groups. We target ≥98% peak purity on every batch.

4
Stage 4 of 9

Mass Spectrometry

Electrospray ionization mass spectrometry (ESI-MS) confirms molecular identity by comparing observed molecular weight against theoretical. Mass deviation under 0.1% is required to pass.

5
Stage 5 of 9

Lyophilization

The purified peptide solution is freeze-dried under controlled vacuum to produce a stable, sterile powder. Lyophilization preserves the peptide's structure and extends shelf life to 24 months at -20°C.

6
Stage 6 of 9

COA Generation

A Certificate of Analysis is created for each lot, including HPLC chromatograms, mass-spec data, lot-specific purity %, molecular weight confirmation, appearance, solubility, and storage conditions. COAs download from every product page.

7
Stage 7 of 9

Sterile Packaging

Lyophilized powder is dispensed into 3 mL borosilicate glass vials under cleanroom conditions. Vials are crimped with tamper-evident aluminum caps and argon-blanketed to prevent oxidation.

8
Stage 8 of 9

Quality Release

A final QA review compares analytical data against pre-defined acceptance criteria. Only lots that pass every specification are released to inventory. Failed lots are quarantined and destroyed under documented SOPs.

9
Stage 9 of 9

Your Order

Orders are picked, inspected against the packing slip, packed with cold packs when appropriate, and shipped within 24 hours of placement. Tracking is provided via email.

Detail 01

HPLC: How We Verify Purity

High-Performance Liquid Chromatography is the gold standard for peptide purity analysis. A small sample of the synthesized peptide is dissolved in a mobile phase and passed through a pressurized C18 column. The column separates the target peptide from impurities based on hydrophobicity. A UV detector at 220 nm records the elution profile as a chromatogram — a graph of absorbance versus time. The area under the main peak, expressed as a percentage of total peak area, is the purity. Every 22EXO batch must achieve ≥98% peak purity to pass. Many batches exceed 99%.

Detail 02

Mass Spectrometry: Confirming Identity

While HPLC tells you how pure the peptide is, mass spectrometry tells you what the peptide is. Electrospray ionization mass spectrometry (ESI-MS) generates a mass-to-charge spectrum from which the molecular weight is deconvoluted. We compare the observed mass against the calculated monoisotopic mass for the target sequence. A deviation greater than 0.1% triggers a reject. This catches misincorporations, incomplete deprotections, and amino acid substitutions that HPLC alone cannot distinguish from the target peptide.

Detail 03

Certificates of Analysis

Every product ships with a downloadable Certificate of Analysis (COA) specific to the lot in your vial. The COA is not a generic template — it contains the actual analytical data from your lot. Contents include: HPLC chromatogram with retention time and peak purity, ESI-MS spectrum with observed and theoretical molecular weights, physical appearance, solubility test results, lot number, synthesis date, and recommended storage conditions. For regulated labs that require chain-of-custody documentation, we can provide extended COAs upon request.

Detail 04

Lyophilization and Stability

Lyophilization (freeze-drying) is the process of removing water from a frozen peptide solution under vacuum. This produces a dry, porous cake that is significantly more stable than the liquid form. Lyophilized peptides stored at -20°C retain their purity for up to 24 months. At 2–8°C (standard refrigerator), stability is maintained for approximately 3 months. Once reconstituted with bacteriostatic water, the solution should be stored at 2–8°C and used within 30 days. We recommend against repeated freeze-thaw cycles, which accelerate degradation.

Detail 05

Cold-Chain Shipping

While lyophilized peptides tolerate ambient temperatures during short transit windows, 22EXO includes cold packs in shipments where weather, transit time, or product sensitivity warrants it. During summer months or for multi-day transit routes, insulated mailers with gel ice packs maintain temperatures below 8°C for up to 48 hours. Express shipping is recommended for temperature-sensitive orders.

Detail 06

Vial and Packaging Standards

22EXO uses 3 mL borosilicate glass vials (USP Type I) — the same glass used in pharmaceutical injectables. Borosilicate is inert and does not leach ions into the peptide solution. Each vial is fitted with a butyl rubber stopper and sealed with a 13 mm aluminum crimp cap. Argon gas is introduced before crimping to displace oxygen and prevent oxidation of methionine and cysteine residues during storage.

Detail 07

Lot Traceability

Every vial carries a lot number that traces back to the specific synthesis run, purification batch, and analytical test records. In the event of a quality concern, this lot number allows us to isolate the batch, review the full production and analytical history, and determine if a corrective action is warranted. We retain analytical records for a minimum of five years.

Detail 08

Quarantine and Release SOPs

Newly synthesized peptides enter a quarantine status in our inventory management system. They cannot be sold or shipped until the full analytical suite (HPLC purity, MS identity, appearance, solubility) has been completed and reviewed by a qualified analyst. If any specification fails, the lot remains in quarantine and is escalated for investigation. Lots that cannot be brought into specification are destroyed with documentation.

See This Process Applied

Every peptide in the catalog ships with the analytical data described above. Browse a few products to see the COA pattern in practice:

  • BPC-157 5mg — The gold-standard cytoprotective pentadecapeptide — 15 amino acids isolated from human gastric juice, studied across 100+ publications for tendon healing, GI protection, and tissue repair.
  • Epithalon 10mg — The telomere peptide — synthetic tetrapeptide that directly activates telomerase (hTERT), extends lifespan by 24% in model organisms, and restores pineal melatonin production.
  • MOTS-C 10mg — The mitochondrial genome peptide — translocates to the cell nucleus under metabolic stress to activate AMPK, improve insulin sensitivity, and mimic the metabolic benefits of exercise.
  • NAD+ 500mg — The cellular energy currency coenzyme — NAD+ levels decline 50% with aging, impairing sirtuins, DNA repair, and mitochondrial function. Research-grade 500mg for NAD+ restoration protocols.
  • BPC-157 + TB-500 Blend (5mg/5mg) — The premier tissue repair combination — 5mg BPC-157 provides localized cytoprotective signaling while 5mg TB-500 delivers systemic angiogenesis and repair signals to remote injury sites.
  • BPC-157 + TB-500 + GHK-Cu Blend (10mg/10mg/50mg) — The ultimate regeneration triple-blend — 10mg BPC-157, 10mg TB-500, and 50mg GHK-Cu combine cytoprotection, systemic angiogenesis, and copper-mediated collagen synthesis for comprehensive tissue repair research.
  • CJC-1295 NO DAC + Ipamorelin Blend (5mg/5mg) — The definitive GH secretagogue combination — CJC-1295 NO DAC loads GH granules via GHRHR while Ipamorelin triggers release via GHS-R1a, producing 3–10x greater GH pulses through dual-receptor synergy.

Explore 22EXO